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課程名稱 |
分子生物學
MOLECULAR BIOLOGY |
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開課學期 |
98-1 |
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授課對象 |
醫學院 病理學研究所 |
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授課教師 |
周綠蘋 |
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課號 |
md&ph5001 |
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課程識別碼 |
420 U0100 |
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班次 |
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學分 |
4 |
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全/半年 |
半年 |
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必/選修 |
必帶 |
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上課時間 |
星期二8,9(15:30~17:20)星期四8,9(15:30~17:20) |
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上課地點 |
基醫301基醫301 |
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備註 |
本課程中文授課,使用英文教科書。與胡務亮、方偉宏、張淑媛、陳培哲、楊偉勛、林榮耀、張明富、張富雄、呂紹俊、李明學、許翠瑛、張 鑫、董馨蓮、陳美如、鄧述諄、李財坤、葉秀慧、錢宗良、李芳仁、阮麗蓉、吳君泰、許秉寧、賈景山、張百恩合開
總人數上限：200人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/981molecular_bio |
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課程簡介影片 |
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核心能力關聯 |
本課程尚未建立核心能力關連 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
見醫衛共同課程時間表 |
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課程目標 |
讓研究基礎醫學的研究生，瞭解原核生物與真核細胞內，核酸與蛋白質
等巨分子的結構與功能，以奠定其醫學研究的基礎。
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課程要求 |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
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評量方式
(僅供參考) |
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週次 |
日期 |
單元主題 |
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第1週 |
9/15,9/17 |
(A) The replicon: unit of replication
（I）Introduction
（II）DNA structure and function
(1)DNA as store of information
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第2週 |
9/22,9/24 |
(A) Outline of major experiments that led to the acceptance of DNA as the genetic material
(B) Structure of nucleic acid, base pairing, tautomeric shift, DNA-A,-B,-Z forms
(C) Denaturing and renaturing of DNA
(D) Supercoiled DNA and topoisomerase
(2) DNA technology, genomic size and genetic content, isolation of DNA by centrifugation and electrophoresis
(3) Replication of DNA
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第3週 |
9/29,10/01 |
(C) Telomere
(B) The apparatus for DNA replication
(4) Genome: organization and evolution |
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第4週 |
10/06,10/08 |
(5) Extrachromosome DNA, nucleosome, chromosome and chromatid
(6) The dynamic genome
(A) Recombination and transposition of DNA in the prokaryotic genome
(B) Rearrangement and amplification in the eukaryotic genome
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第5週 |
10/13,10/15 |
（III）Transcription, regulation and processing
(1) Transcription and regulation (prokaryotic RNA pol. and eukaryotic RNA pol. II)
(A) Transcriptional Regulation in Eukaryotic Chromatin I
(B) Transcriptional Regulation in Eukaryotic Chromatin II
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第6週 |
10/20,10/22 |
期中考；(7) DNA damage and mutation |
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第7週 |
10/27,10/29 |
(8) DNA repair and modification
(2) Transcription and regulation (eukaryotic RNA pol. I and III)
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第8週 |
11/03,11/05 |
(3) Post-transcriptional processing, ribozyme and regulation
(4) Phage strategies : control of lytic and lysogenic pathway
(5) A brief introduction to animal viruses
(6) Phage display library
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第9週 |
11/10,11/12 |
（IV）Translation
(1) Assembly line for protein synthesis
(2) Transfer RNA is the translated adaptor
(3) Ribosomes provide a translation factory
(4) Codons and their significance
(5) Translation in cell free system
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第10週 |
11/17,11/19 |
(6) Post-translational modification
I. Structure
II. Mechanism and protein degradation
（V）Recombinant DNA technology（I）
(1) Cloning of DNA：transformation and screening of transformants
(2) Construction of cDNA and genomic libraries
(3) Colony and plaque hybridization, Southern blot, Northern blot, Western blot analysis
(4) Primer extension, S1 nuclease mapping and RNase protection assay
(5) Differential display
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第11週 |
11/24,11/26 |
(6) Site-directed mutagenesis and polymerase chain reaction
(7) Application of restriction fragment length polymorphism and variable number of tandem repeat for genetic disease analysis
(8) Genome-wide sequencing strategies
第二次期中考(考試範圍10/22~11/24)
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第12週 |
12/01,12/03 |
（VI）Construction of the cells
(1) Signal transduction in prokaryotes
(2) Signal transduction in eukaryotes
（VII）Genes in the development
(1) Immune diversity and immunotherapy
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第13週 |
12/08,12/10 |
(2) Gene regulation and development, RNA interference, micro RNA
(3) Oncogenes
(4) Tumor suppressor genes
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第14週 |
12/15,12/17 |
（IX）Recombinant DNA technology（II）
(2) Yeast genetics and its applications
(1) Expression of cloned gene in prokaryotic and eukaryotic cells
(3) Applications of bioinformatics and genomics in biomedical research
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第15週 |
12/22,12/24 |
(4) Current development of genome projects and their impacts to
human diseases
(5) Transgenic techniques
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第16週 |
12/29 |
(6) Gene therapy |
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第17週 |
1/05 |
期末考 (考試範圍12/01~12/29) |
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